Sharps injuries are one of the most common incidents reported in research laboratories. Injuries from a cryostat or microtome blade can be significant.
The following practices should be used when sectioning:
- The blade, handwheel, and foot pedal should be locked and the blade guard (if available) should be in place before any manipulation of materials.
- The foot pedal should have a cover or guard, or it should be positioned to prevent unintended activation.
- Install and remove blades using forceps or a magnetic tool. Never remove blades with fingers, and wear cut resistant gloves when installing or removing blades.
- Use tools when removing slices or ribbons, or when wiping the block/plate. Hands and fingers should remain clear of the blade when working with materials.
- Wear cut-resistant gloves when cleaning the equipment.
- Schedule regular breaks to prevent injuries due to fatigue.
Additional information on cryostat and microtome safety can be found online.
The Office of Biological Safety is happy to answer any questions or provide guidance. Please contact us if you have any questions or concerns: biosafety@fpm.wisc.edu, 608-263-2037.
by Karen Demick
Karen Demick is a Biosafety Specialist on the IBC and Laboratory Operations team. She joined EH&S in 2011 and supports the research community through reviewing biosafety protocols and outreach to laboratories.
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